Glutamate Decarboxylase fromLactobacillus brevis: Activation by Ammonium Sulfate

Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate.

In this study, the glutamate decarboxylase (GAD) gene from Lactobacillus brevis IFO12005 (Biosci. Biotechnol. Biochem., 61, 1168-1171 (1997)), was cloned and expressed. The deduced amino acid sequence showed 99.6% and 53.1% identity with GAD of L. brevis ATCC367 and L. lactis respectively. The His-tagged recombinant GAD showed an optimum pH of 4.5-5.0, and 54 kDa on SDS-PAGE. The GAD activity a...

cloning of glutamate decarboxylase and production of γ-aminobutyric acid from lactobacillus brevis

Mammalian phospholipase D: activation by ammonium sulfate and nucleotides.

Phospholipase D (PLD) associated with the rat kidney membrane was activated by guanine 5'-[gamma-thio]triphosphate and a cytosol fraction that contained ADP-ribosylation factor. When assayed by measuring the phosphatidyl transfer reaction to ethanol with exogenously added radioactive phosphatidylcholine as substrate, the PLD required a high concentration (1.6 M) of ammonium sulfate to exhibit h...

Brain Glutamate Decarboxylase

Glutamate decarboxylase (glutamate 1-carboxylyase, EC 4.1.1.15, GAD)* IS the major, rate-limiting enzyme in brain for synthesizing gamma-aminobutyric acid (GABA). Total GAD activity in brain is 10-20 times greater than the observed rate of GABA synthesis (Collins, 1972; Matsui and Deguchi, 1977; Casu and Gale, 1981), indicating that GAD operates at only a fraction of its capacity. Although othe...

Ammonium sulfate – malonic acid aerosols

A study of the phase transition behavior of mixed ammonium sulfate – malonic acid aerosols C. F. Braban and J. P. D. Abbatt Centre for Atmospheric Sciences, Department of Chemistry, Lensfield Road, University of Cambridge, Cambridge, CB2 1EW, UK Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada Received: 22 March 2004 – Accepted: 3 May 2004 – Published: 2 June 2004 Co...